NR1H2 Knockout cell line (Vips)

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NR1H2 Knockout cell line (Vips) Order

NR1H2 Knockout cell line  (Vips)
Catalog#
YKO-H879
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NR1H2 Knockout cell line  (Vips)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NR1H2 Knockout cell line (Vips)
Species
Human
Gene Name
NR1H2
Gene ID
7376
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3,Exon 4 (based on transcript NR1H2-201)
Validation: PCR+Sanger Sequencing
NR1H2 Knockout cell line  (Vips) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NR1H2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Vips cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NR1H2 gene knockout NR1H2 Knockout cell line (Vips) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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